Differential expression of microRNAs in the saliva of patients with aggressive periodontitis: a pilot study of potential biomarkers for aggressive periodontitis
이남훈, 이은혜, 김영성, 김원경, 이영규, 김수환,
소속 상세정보
이남훈 ( Lee Nam-Hun ) - Asan Medical Center Department of Periodontics
이은혜 ( Lee Eun-Hye ) - Seoul National University School of Dentistry Department of Conservative Dentistry
김영성 ( Kim Young-Sung ) - Asan Medical Center Department of Periodontics
김원경 ( Kim Won-Kyung ) - Asan Medical Center Department of Periodontics
이영규 ( Lee Young-Kyoo ) - Asan Medical Center Department of Periodontics
김수환 ( Kim Su-Hwan ) - Asan Medical Center Department of Periodontics
Abstract
Purpose: The aim of this study was to compare microRNA (miRNA) gene expression in saliva using miRNA polymerase chain reaction (PCR) arrays in healthy and aggressive periodontitis (AP) patients.
Methods: PCR arrays of 84 miRNAs related to the human inflammatory response and autoimmunity from the saliva samples of 4 patients with AP and 4 healthy controls were performed. The functions and diseases related to the miRNAs were obtained using TAM 2.0. Experimentally validated targets of differentially expressed miRNAs were obtained from mirTarBase. Gene ontology terms and pathways were analyzed using ConsensusPathDB.
Results: Four downregulated miRNAs (hsa-let-7a-5p, hsa-let-7f-5p, hsa-miR-181b-5p, and hsa-miR-23b-3p) were identified in patients with AP. These miRNAs are associated with cell death and innate immunity, and they target genes associated with osteoclast development and function.
Conclusions: This study is the first analysis of miRNAs in the saliva of patients with AP. Identifying discriminatory human salivary miRNA biomarkers reflective of periodontal disease in a non-invasive screening assay is crucial for the development of salivary diagnostics. These data provide a first step towards the discovery of key salivary miRNA biomarkers for AP.
키워드
Aggressive periodontitis; Biomarkers; MicroRNAs; Saliva
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